Spectrin beta Chain, Erythrocyte (SPTB) antibody
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- Target See all Spectrin beta Chain, Erythrocyte (SPTB) Antibodies
- Spectrin beta Chain, Erythrocyte (SPTB)
- Reactivity
- Human, Mouse, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Coating (Coat), ELISA, Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogen
- Red cell membranes
- Clone
- RG-26
- Isotype
- IgG2a kappa
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- Application Notes
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Positive Control: Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), ,Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Spectrin beta Chain, Erythrocyte (SPTB)
- Alternative Name
- SPTB (SPTB Products)
- Synonyms
- AI842465 antibody, D330027P03Rik antibody, Gm1301 antibody, Spnb-1 antibody, Spnb1 antibody, ja antibody, jaundiced antibody, mKIAA4219 antibody, EL3 antibody, HS2 antibody, HSPTB1 antibody, SPH2 antibody, spectrin beta, erythrocytic antibody, spectrin, beta, erythrocytic antibody, SPTB antibody, Sptb antibody
- Background
- Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.
- Molecular Weight
- 246kDa
- Gene ID
- 6710
- UniProt
- O15020
- Pathways
- Regulation of Actin Filament Polymerization
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