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S100A8 antibody

S100A8 Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Cat, Dog, Monkey, Horse, Guinea Pig, Baboon, Goat IHC, IF, FACS, StM Host: Mouse Monoclonal MRP8 7C12-4 unconjugated
Catalog No. ABIN6940546
  • Target See all S100A8 Antibodies
    S100A8 (S100 Calcium Binding Protein A8 (S100A8))
    Reactivity
    Human, Mouse, Rat, Cow, Pig, Rabbit, Cat, Dog, Monkey, Horse, Guinea Pig, Baboon, Goat
    Host
    • 92
    • 35
    • 3
    • 3
    • 1
    Mouse
    Clonality
    • 97
    • 37
    Monoclonal
    Conjugate
    • 61
    • 21
    • 17
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This S100A8 antibody is un-conjugated
    Application
    • 84
    • 47
    • 42
    • 28
    • 16
    • 13
    • 13
    • 13
    • 12
    • 11
    • 7
    • 6
    • 6
    • 6
    • 3
    • 2
    • 1
    Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
    Specificity
    Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12 kDa alpha chain and a 14 kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
    Purification
    Purified by Protein A/G
    Immunogen
    Affinity Purified monocyte membrane preparation
    Clone
    MRP8 7C12-4
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product S100A8 Primary Antibody
  • Application Notes

    Positive Control: Tonsil, lymph node or spleen.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    S100A8 (S100 Calcium Binding Protein A8 (S100A8))
    Alternative Name
    S100A8 (S100A8 Products)
    Synonyms
    60B8AG antibody, CAGA antibody, CFAG antibody, CGLA antibody, CP-10 antibody, L1Ag antibody, MA387 antibody, MIF antibody, MRP8 antibody, NIF antibody, P8 antibody, Mrp8 antibody, S100A8 antibody, 60B8Ag antibody, AI323541 antibody, B8Ag antibody, CFAg antibody, Caga antibody, p8 antibody, MRP-8 antibody, S100 calcium binding protein A8 antibody, S100 calcium binding protein A8 (calgranulin A) antibody, S100A8 antibody, S100a8 antibody
    Molecular Weight
    MAC387
    Gene ID
    6279, 6280
    UniProt
    P05109, P06702
    Pathways
    Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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