PPP3R1 antibody

Catalog No. ABIN6940383

Product Details anti-PPP3R1 Antibody

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Reactivity: Human, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPP3R1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Staining Methods (StM), Coating (Coat)

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human Calcineurin B protein

Clone: BE2-1

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: HeLa, MCF-7 or A431 cells. Human brain, breast or stomach tissue.

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PPP3R1

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Alternative Name: PPP3R1 (PPP3R1 Products)

Synonyms: si:ch211-274c22.8 antibody, canb antibody, CALNB1 antibody, CNB antibody, CNB1 antibody, CaNB1 antibody, Cnb1 antibody, MCIP1 antibody, protein phosphatase 3, regulatory subunit B, alpha a antibody, protein phosphatase 3 regulatory subunit B, alpha S homeolog antibody, protein phosphatase 3 regulatory subunit B, alpha antibody, protein phosphatase 3, regulatory subunit B, alpha antibody, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) antibody, ppp3r1a antibody, ppp3r1.S antibody, PPP3R1 antibody, Ppp3r1 antibody

Background: Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59 kDa catalytic A subunit and a 19 kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Molecular Weight: 19kDa

Gene ID: 5534

UniProt: P63098

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling