Recombinant ATRX antibody

Catalog No. ABIN6940363

Product Details anti-ATRX Antibody

Target: ATRX (helicase 2, X-linked (ATRX))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ATRX antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Recombinant human ATRX protein

Clone: ATRX-2900R

Isotype: IgG

Application Details

Application Notes:

Positive Control: Raji, HeLa or A431 cells. Pancreas, Adrenal, Ovary, Kidney, Prostate or Stomach.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ATRX

Target: ATRX (helicase 2, X-linked (ATRX))

Alternative Name: ATRX (ATRX Products)

Background: ATRX is a member of the Snf2 family of helicase/ATPases, which contribute to the remodeling of the nucelosome structure in an ATP-dependent manner, and facilitate the initiation of transcription and replication. Structurally, ATRX contains a PHD zinc finger motif. ATRX is regulated throughout the cell cycle where it is differentially distributed within the nucleus. During interphase, ATRX predominately associates with the nuclear matrix, while during mitosis, ATRX localizes with condensed chromatin. At the onset of M phase, phosphorylation rapidly induces this redistribution of ATRX to the short arms of human acrocentric chromosomes, where it then specifically complexes with heterochromatin protein 1 thalassemia or ATRX syndrome

Molecular Weight: 280kDa

Gene ID: 546

UniProt: P46100