PAX2A antibody (AA 223-354)

Catalog No. ABIN6940252

Product Details anti-PAX2A Antibody

Target: PAX2A (Paired Box Gene 2a (PAX2A))

Binding Specificity: AA 223-354

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX2A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)

Specificity: Recognizes a protein of 42 kDa, which is identified as PAX2. It is a member of the paired box family of transcription factors, which is required for development and proliferation of the kidney, brain, and mullerian organs. PAX2 genes contain a highly conserved DNA sequence within the paired box region, which encodes a DNA-binding domain, enabling PAX proteins to bind the promoters of specific genes to transcriptionally regulate their expression. PAX2 is specifically expressed in the developing central nervous system, eye, ear, and urogenital tract, and is essential for the development of these organs. In normal adult tissues PAX2 was mainly detected in the urogenital system, including kidney, ureteric epithelium, fallopian tube epithelium, ovary and uterus. In tumors, PAX2 has been detected in renal cell carcinomas, Wilms' tumors, nephrogenic adenomas and papillary serous carcinoma of the ovary. PAX2 has been used as a marker for the identification of renal cell carcinoma and ovarian carcinoma by immunohistochemistry.

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 223-354) human PAX2 protein (exact sequence is proprietary)

Clone: PAX2-1104

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: NAMALWA cells. Fetal kidney or Renal Cell Carcinoma (RCC) or Ovarian Carcinoma.

Known Application: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PAX2A

Target: PAX2A (Paired Box Gene 2a (PAX2A))

Alternative Name: PAX2 (PAX2A Products)

Synonyms: PAPRS antibody, Opdc antibody, Pax-2 antibody, Pax-2a antibody, XPax-2 antibody, XPax2 antibody, pax-2 antibody, pax2 antibody, xPax-2a antibody, PAXZF-B antibody, cb378 antibody, noi antibody, pax-b antibody, pax2.1 antibody, pax2a1 antibody, pax[zf-b] antibody, paxb antibody, pax2.2 antibody, paired box 2 antibody, paired box 2 L homeolog antibody, paired box 2a antibody, paired box 2b antibody, PAX2 antibody, Pax2 antibody, pax2.L antibody, pax2a antibody, pax2b antibody

Molecular Weight: 42kDa

Gene ID: 5076

UniProt: Q02962

Pathways: Carbohydrate Homeostasis, Stem Cell Maintenance, Tube Formation