Myogenin antibody (AA 30-224)
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- Target See all Myogenin (MYOG) Antibodies
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
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Binding Specificity
- AA 30-224
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Reactivity
- Human, Mouse, Rat, Pig, Cat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Myogenin antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogen
- Human myogenin recombinant protein (MGN185), Rat myogenin recombinant fragment containing amino acid 30-224 (F5D)
- Clone
- MGN185-F5D
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product MYOG Primary Antibody
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- Application Notes
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Positive Control: Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
- Alternative Name
- MYOG (MYOG Products)
- Synonyms
- cb553 antibody, zgc:100763 antibody, LOC446037 antibody, myf4 antibody, Xmyog antibody, myogenin antibody, LOC100136471 antibody, LOC100224062 antibody, LOC100303673 antibody, MYF4 antibody, bHLHc3 antibody, myo antibody, myf-4 antibody, myogenin antibody, myogenin (myogenic factor 4) antibody, MYOG antibody, myog antibody, LOC100303673 antibody, Myog antibody
- Background
- Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80 % amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.
- Molecular Weight
- 34kDa
- Gene ID
- 4656
- UniProt
- P15173
- Pathways
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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