Recombinant MTAP antibody (AA 97-196)

Catalog No. ABIN6940088

Product Details anti-MTAP Antibody

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 97-196

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MTAP antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM), Immunostaining (ISt)

Specificity: Recognizes a protein of 31 kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.

Purification: Purified by Protein A/G

Immunogen: Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary)

Clone: RMTAP-1813

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HepG2, A431, HeLa or MCF-7 cells. Human kidney tissues (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MTAP

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Alternative Name: MTAP (MTAP Products)

Molecular Weight: 31kDa

Gene ID: 4507

UniProt: Q13126

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process