Moesin antibody

Catalog No. ABIN6940085

Product Details anti-Moesin Antibody

Target: Moesin (MSN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Moesin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM), Blocking Peptide (BP)

Specificity: Recognizes 78 kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.

No Cross-Reactivity: Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human Moesin protein

Clone: MSN-493

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: K562, HT-29, Jurkat, CH3LC or HUVEC cells. Uterus, placenta, tonsil (both B and T lymphocytes), skeletal muscle, thyroid or kidney.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (2-4 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Moesin

Target: Moesin (MSN)

Alternative Name: MSN (MSN Products)

Synonyms: CG10701 antibody, D17 antibody, Dmel\\CG10701 antibody, Dmoe antibody, EMR1 antibody, ERM antibody, Emr1 antibody, anon-WO03040301.155 antibody, anon-WO03040301.157 antibody, anon-WO03040301.159 antibody, anon-WO03040301.161 antibody, dMoe antibody, gma antibody, l(1)G0067 antibody, l(1)G0323 antibody, l(1)G0404 antibody, l(1)G0415 antibody, moe antibody, moesin/ezrin/radixin homolog mRNA antibody, cb567 antibody, rdx antibody, wu:fb14b03 antibody, zgc:85640 antibody, MSN antibody, C78546 antibody, moesin antibody, Moesin antibody, moesin a antibody, moesin L homeolog antibody, MSN antibody, Moe antibody, msna antibody, msn.L antibody, moes antibody, Msn antibody

Molecular Weight: 78kDa

Gene ID: 4478

UniProt: P26038

Pathways: Asymmetric Protein Localization