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Recombinant EpCAM antibody (Extracellular Domain)

Name: Recombinant EpCAM antibody (Extracellular Domain)
Brand: antibodies-online
SKU: ABIN6939997
Price: 618.92 USD
Availability: InStock

EPCAM Reactivity: Human FACS, IHC, StM Host: Rabbit Monoclonal EGP40-2571R unconjugated Recombinant Antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2571R). (Recombinant EpCAM antibody (Extracellular Domain))

IHC

SDS-PAGE Analysis Purified EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2571R). (Recombinant EpCAM antibody (Extracellular Domain))

SDS

Flow Cytometric Analysis of MCF-7 cells using EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2571R) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red). (Recombinant EpCAM antibody (Extracellular Domain))

FACS

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2571R). (Recombinant EpCAM antibody (Extracellular Domain))

IHC

4 images

Catalog No. ABIN6939997

Datasheet as PDF

Target See all EpCAM (EPCAM) Antibodies

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antibody Type

Recombinant Antibody
Binding Specificity
56

42

30

27

16

16

14

12

12

11

10

8

5

4

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 100-224, Extracellular Domain
Reactivity
291

78

37

2

1

1
Human
Host
153

146

11

1

1
Rabbit
Compatible Secondaries
Clonality
191

116

3
Monoclonal
Conjugate
153

21

18

11

9

8

8

8

8

8

8

5

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1
This EpCAM antibody is un-conjugated
Application
211

180

121

112

84

33

26

25

24

20

17

16

15

11

3

3

2

2

2

1

1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)

Specificity

Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Immunogen

Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary)

Clone

EGP40-2571R

Isotype

IgG

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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 25-265) antibody
EPCAM Reactivity: Pig WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 53-150) antibody
EPCAM Reactivity: Human, Mouse, Rat, Rabbit WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibody
EPCAM Reactivity: Human WB, FACS, IHC, ELISA Host: Mouse Monoclonal 7B5C10 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reactivity: Human WB, FACS, IF, IHC, StM Host: Mouse Monoclonal EGP40-1373 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal rEGP40-1372 unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reactivity: Human FACS, IF, IHC, ELISA, StM, Coat Host: Mouse Monoclonal EGP40-1384 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reactivity: Human WB, FACS, IF, IHC, StM Host: Mouse Monoclonal EGP40-1372 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 20-60), (N-Term) antibody
EPCAM Reactivity: Human FACS, IHC, StM Host: Rabbit Monoclonal EGP40-1555R unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 280-350), (Cytoplasmic Domain) antibody
EPCAM Reactivity: Human, Mouse, Rat IHC, StM Host: Mouse Monoclonal EGP40-1110 unconjugated

Application Notes

Positive Control: MCF-7 or HT29 cells. Breast tumor.
Known Application: Flow Cytometry (1-2 μg/million cells). Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions

For Research Use only
Concentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-80 °C

Storage Comment

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date

24 months
Target

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name

TACSTD1 (EPCAM Products)

Molecular Weight

40-43kDa

Gene ID

4072

UniProt

P16422