Recombinant EpCAM antibody (Extracellular Domain)
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- Target See all EpCAM (EPCAM) Antibodies
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- Antibody Type
- Recombinant Antibody
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Binding Specificity
- AA 77-202, Extracellular Domain
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This EpCAM antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant human EpCAM protein fragment from extracellular domain (around aa77-202) (exact sequence is proprietary)
- Clone
- REGP40-1372
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product EPCAM Primary Antibody
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- Application Notes
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Positive Control: HT29 cells. Ovarian carcinoma.
Known Application: Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- Alternative Name
- TACSTD1 (EPCAM Products)
- Background
- EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
- Molecular Weight
- 40-43kDa
- Gene ID
- 4072
- UniProt
- P16422
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