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CD25 antibody (AA 42-183)

IL2RA Reactivity: Human IHC, StM Host: Mouse Monoclonal IL2RA-2395 unconjugated
Catalog No. ABIN6939779
  • Target See all CD25 (IL2RA) Antibodies
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    Binding Specificity
    • 16
    • 10
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 42-183
    Reactivity
    • 175
    • 110
    • 31
    • 9
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 129
    • 85
    • 77
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 212
    • 79
    • 2
    Monoclonal
    Conjugate
    • 106
    • 32
    • 26
    • 26
    • 21
    • 14
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD25 antibody is un-conjugated
    Application
    • 207
    • 85
    • 51
    • 49
    • 39
    • 36
    • 19
    • 16
    • 14
    • 14
    • 8
    • 8
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Staining Methods (StM)
    Specificity
    Recognizes a protein of 55 kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin's disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 Molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant fragment of human IL2RA protein (around aa 42-183) (exact sequence is proprietary)
    Clone
    IL2RA-2395
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product IL2RA Primary Antibody
  • Application Notes

    Positive Control: PHA-stimulated human lymphocytes. Human lymph node or tonsil.

    Known Application: Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    Alternative Name
    IL2RA (IL2RA Products)
    Synonyms
    CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody
    Molecular Weight
    55kDa
    Gene ID
    3559
    UniProt
    P01589
    Pathways
    JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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