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IgL antibody

Reactivity: Human IHC, FACS, IF, WB, StM Host: Mouse Monoclonal N10-2 unconjugated
Catalog No. ABIN6939763
  • Target See all IgL products
    IgL
    Reactivity
    Human
    Host
    • 9
    • 3
    Mouse
    Clonality
    • 11
    • 1
    Monoclonal
    Conjugate
    • 11
    • 1
    This IgL antibody is un-conjugated
    Application
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Staining Methods (StM)
    Specificity
    This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
    Purification
    Purified by Protein A/G
    Immunogen
    Purified IgG from human serum
    Clone
    N10-2
    Isotype
    IgG1 kappa
  • Application Notes

    Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

    Known Application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    IgL
    Abstract
    IgL Products
    Synonyms
    IGL@ antibody, IGLC6 antibody, igl antibody, immunoglobulin lambda locus antibody, IGL antibody, igl@ antibody
    Molecular Weight
    ~22.5kDa
    Gene ID
    3535, 3546
    UniProt
    P01701, P01842
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