IGKC antibody

Catalog No. ABIN6939745

Product Details anti-IGKC Antibody

Target: IGKC (Immunoglobulin kappa Constant (IGKC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGKC antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB), Flow Cytometry (FACS)

Specificity: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Purification: Purified by Protein A/G

Immunogen: Recombinant human Ig kappa chain

Clone: KLC264

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IGKC

Target: IGKC (Immunoglobulin kappa Constant (IGKC))

Alternative Name: IGKC (IGKC Products)

Synonyms: HCAK1 antibody, IGKCD antibody, Km antibody, IGKC antibody, Igk-C antibody, immunoglobulin kappa constant antibody, immunoglobulin kappa constant L homeolog antibody, IGKC antibody, LOC708048 antibody, igkc antibody, igkc.L antibody, Igkc antibody

Molecular Weight: ~22.5kDa

Gene ID: 3514

UniProt: P01601, P01834