IgD antibody

Catalog No. ABIN6939707

Product Details anti-IgD Antibody

Target: IgD

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgD antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Full-length human IGHD protein

Clone: IgD26

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Human tonsil lysate, NAMALWA cell lysate, Human tonsil and lymphoid tissues.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IgD

Target: IgD

Alternative Name: IGHD (IgD Products)

Synonyms: IgD antibody, Igh-5 antibody, immunoglobulin heavy constant delta antibody, immunoglobulin delta heavy chain constant region antibody, Ighd antibody, Igd antibody, IGHD antibody

Target Type: Antibody

Background: Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin D (IgD) exists as a monomer with light chains. It plays a biological role as a transmembrane receptor molecule, co-expressed with IgM on the surface of mature/naive B cells. In particular, it is found on spleen B cell surfaces. Compared to IgM, IgD exists in much lower numbers and is not expressed on immature B cells. IgD surface expression on B cells is regulated in part by IL-27. In mice, the inhibition of this immunoglobulin isotype does not cause a significant change to the immune system.

Molecular Weight: 44-80kDa (heavy chain), 21-25kDa (kappa light chain)

Gene ID: 3495

UniProt: P01880