SHP1 antibody

Catalog No. ABIN6939660

Product Details anti-SHP1 Antibody

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Recombinant human full-length APEX1 protein

Clone: CPTC-APEX1-2

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: A431, A549, PC3, HAP1, HePG2, MCF-7, HeLa, NIH/3T3 and C6 whole cell lysates. Human ovarian carcinoma.

Known Application: Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SHP1

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Alternative Name: PTPN6 (PTPN6 Products)

Synonyms: HCPH antibody, HPTP1C antibody, PTP-1C antibody, SH-PTP1 antibody, SHP-1 antibody, SHP-1L antibody, SHP1 antibody, DRPLA antibody, Hcph antibody, Ptp1C antibody, me antibody, motheaten antibody, Ptph6 antibody, Shp-1 antibody, fj22f05 antibody, wu:fj22f05 antibody, zgc:55501 antibody, ptpn6 antibody, protein tyrosine phosphatase, non-receptor type 6 antibody, protein tyrosine phosphatase, non-receptor type 6 L homeolog antibody, PTPN6 antibody, Ptpn6 antibody, ptpn6 antibody, ptpn6.L antibody

Background: APEX / APE1 is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Patients with genetic variants in APEX1 and XRCC1 have been shown to have a higher risk of lung cancer. Elevated APEX1 levels observed in human testicular cancer may be related to relative resistance to therapy and therefore may serve as a diagnostic marker for refractory disease.

Molecular Weight: 35kDa

Gene ID: 328

UniProt: P27695

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling