HLA-DRB1 antibody
-
- Target See all HLA-DRB1 Antibodies
- HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))
-
Reactivity
- Human, Monkey, Baboon, Chimpanzee, Dog, Squirrel
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This HLA-DRB1 antibody is un-conjugated
-
Application
- Flow Cytometry (FACS), Immunofluorescence (IF)
- Specificity
- This MAb reacts with the HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. It binds a conformational epitope on HLA-DR, which depends on the correct folding of the heterodimer. This MAb has been reported to block mixed lymphocyte reactions. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kD alpha (heavy) chain and a 28kD beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
- Purification
- Purified by Protein A/G
- Immunogen
- Activated human peripheral blood mononuclear cells
- Clone
- L243
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product HLA-DRB1 Primary Antibody
-
-
- Application Notes
-
Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
-
- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
-
- Target
- HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))
- Alternative Name
- HLA-DRB1 (HLA-DRB1 Products)
- Synonyms
- MGC108235 antibody, DRB1 antibody, DRw10 antibody, HLA-DR1B antibody, HLA-DRB antibody, SS1 antibody, major histocompatibility complex, class II, DR beta 1 antibody, hla-drb1 antibody, HLA-DRB1 antibody
- Molecular Weight
- ~28kDa (beta chain)
- Gene ID
- 3123
- UniProt
- P01911
- Pathways
- TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
-