HLA-DR antibody

Catalog No. ABIN6939641

Product Details anti-HLA-DR Antibody

Target: HLA-DR

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DR antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt), Staining Methods (StM)

Specificity: This MAb reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Purification: Purified by Protein A/G

Immunogen: Activated human peripheral blood mononuclear cells

Clone: SPM289

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: Raji, Ramos, Daudi or HuT78 cells. Spleen, Tonsil or lymph node.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (2-4 μg/mL), Western Blot (0.5-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HLA-DR

Target: HLA-DR

Alternative Name: HLA-DR (HLA-DR Products)

Molecular Weight: ~28kDa (beta chain)

Gene ID: 3123

UniProt: P01903

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response