Recombinant HLA DQ antibody

Catalog No. ABIN6939633

Product Details anti-HLA DQ Antibody

Target: HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))

Antibody Type: Recombinant Antibody

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA DQ antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.

Purification: Purified by Protein A/G

Immunogen: T4-positive CTL clone HG-38

Clone: RSPV-L3

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Raji cells. Tonsil or lymph node.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Frozen only) (0.5-1 μg/mL for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HLA DQ

Target: HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))

Alternative Name: HLA-DQ (HLA-DQ Products)

Molecular Weight: 60kDa

Gene ID: 3117, 3118, 3119

UniProt: P01908, P01909, P01920