MHC Class II HLA-DP/DQ/DR antibody
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- Target See all MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR) products
- MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MHC Class II HLA-DP/DQ/DR antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), Coating (Coat), ELISA, Staining Methods (StM)
- Specificity
- Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
- Purification
- Purified by Protein A/G
- Immunogen
- Cells from human tonsil
- Clone
- CR3-43
- Isotype
- IgG1 kappa
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- Application Notes
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Positive Control: Ramos, Raji cells. Tonsil, Spleen or lymph node.
Known Application: ELISA (For coating use Ab at 2-4 μg/mL, order Ab without BSA),Flow Cytometry (1-2 μg/ million cells), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10mM PBS with 0.05% BSA & 0.05% azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
- Alternative Name
- HLA-DP, HLA-DQ, HLA-DR (HLA-DP/DQ/DR Products)
- Molecular Weight
- 36kDa (' chain) and 27kDa (' chain)
- Gene ID
- 3115, 3117, 3122
- UniProt
- P04440, P01908, P01909, P01920, P01903
- Pathways
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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