GZMB antibody (AA 73-187)

Catalog No. ABIN6939593

Product Details anti-GZMB Antibody

Target: GZMB (Granzyme B (GZMB))

Binding Specificity: AA 73-187

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human GZMB protein (around aa 73-187) (exact sequence is proprietary)

Clone: GZMB-3056

Isotype: IgG kappa

Application Details

Application Notes:

Positive Control: A431 cells. Human tonsil, spleen or Hodgkin's Lymphoma tissue (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: GZMB (GZMB Products)

Synonyms: CCPI antibody, CGL-1 antibody, CGL1 antibody, CSP-B antibody, CSPB antibody, CTLA1 antibody, CTSGL1 antibody, HLP antibody, SECT antibody, granzyme B antibody, GZMB antibody

Background: Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.

Molecular Weight: 29-32kDa

Gene ID: 3002

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis