MSH6 antibody (AA 374-540)

Catalog No. ABIN6939576

Product Details anti-MSH6 Antibody

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Binding Specificity: AA 374-540

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human MSH6 protein (around aa 374-540) (exact sequence is proprietary)

Clone: MSH6-3091

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: HCT116, MCF-7 cells, Human colon carcinoma (IHC).

Known Application: ELISA (For coating, order Ab without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Alternative Name: MSH6 (MSH6 Products)

Synonyms: GTBP antibody, GTMBP antibody, HNPCC5 antibody, HSAP antibody, p160 antibody, cb326 antibody, wu:fb36c12 antibody, LOC100227810 antibody, AU044881 antibody, AW550279 antibody, Gtmbp antibody, mutS homolog 6 antibody, mutS homolog 6 (E. coli) antibody, DNA mismatch repair protein antibody, MSH6 antibody, msh6 antibody, Msh6 antibody

Background: The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.

Molecular Weight: 163kDa

Gene ID: 2956

UniProt: P52701

Pathways: DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response