PD-L1 antibody (AA 39-191)

Catalog No. ABIN6939571

Product Details anti-PD-L1 Antibody

Target: PD-L1 (CD274 (PD-L1))

Binding Specificity: AA 39-191

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)

Clone: PDL1-2746

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: Heart, Placenta, Spleen or Tonsil tissue. RAW, HEK293 or HepG2 cell lysates. Jurkat cells.

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274 (PD-L1 Products)

Synonyms: B7-H antibody, B7H1 antibody, PD-L1 antibody, PDCD1L1 antibody, PDCD1LG1 antibody, PDL1 antibody, A530045L16Rik antibody, B7h1 antibody, Pdcd1l1 antibody, Pdcd1lg1 antibody, Pdl1 antibody, RGD1566211 antibody, CD274 molecule antibody, CD274 antigen antibody, programmed cell death 1 ligand 2 antibody, CD274 antibody, Cd274 antibody, PDCD1LG2 antibody

Background: PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

Molecular Weight: 37-50kDa

Gene ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints