LGALS13 antibody (AA 23-134)

Catalog No. ABIN6939562

Product Details anti-LGALS13 Antibody

Target: LGALS13 (Lectin, Galactoside-Binding, Soluble, 13 (LGALS13))

Binding Specificity: AA 23-134

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LGALS13 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Staining Methods (StM)

Specificity: It recognizes a 32 kDa protein, which is identified as homodimer of galectin-13 (also known as PP13). Galectins are a family of soluble -galactoside-binding lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One such member, galectin-13, is a 139 amino acid protein that contains one galectin domain. Possessing lysophospholipase activity, galectin-13 exists as a disulfide-linked homodimer. Galectin-13 is suggested to have a developmental role in the placenta and may display immunobiological activity in feto-maternal blood-spaces. Screening for galectin-13 in maternal serum during the first trimester of pregnancy may serve as a diagnostic marker to predict preterm pre-eclampsia.

Purification: Purified by Protein A/G

Immunogen: Recombinant human Galectin-13 protein fragment (aa23-134)

Clone: PP13-1161

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: K-562 cells. Placenta or Spleen.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 or 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for LGALS13

Target: LGALS13 (Lectin, Galactoside-Binding, Soluble, 13 (LGALS13))

Alternative Name: LGALS13 (LGALS13 Products)

Synonyms: GAL13 antibody, PLAC8 antibody, PP13 antibody, Gal-11 antibody, Gal-13 antibody, Gal11 antibody, gal-13 antibody, galectin 13 antibody, avian beta-defensin 13 antibody, LGALS13 antibody, AvBD13 antibody

Molecular Weight: 16kDa (monomer), 32kDa (homodimer)

Gene ID: 29124

UniProt: Q9UHV8