ABO antibody

Catalog No. ABIN6939553

Product Details anti-ABO Antibody

Target: ABO (Histo-blood group A transferase (ABO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ABO antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Agglutination (AGG), Haemagglutination (H), Staining Methods (StM)

Specificity: This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Immunogen: Mixture of erythrocytes of blood group Aand glyco protein fraction isolated from the saliva of secretors with blood group A

Clone: HE-193

Isotype: IgM kappa

Application Details

Application Notes:

Positive Control: KG1 cells. Human colorectal carcinoma tissues.

Known Application: Agglutination, Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ABO

Target: ABO (Histo-blood group A transferase (ABO))

Alternative Name: ABO (ABO Products)

Synonyms: A3GALNT antibody, A3GALT1 antibody, GTB antibody, NAGAT antibody, ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase antibody, ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase, transferase B, alpha 1-3-galactosyltransferase) antibody, ABO antibody, Abo antibody

Gene ID: 28

UniProt: P16442