14-3-3 sigma/SFN antibody

Catalog No. ABIN6939540

Product Details anti-14-3-3 sigma/SFN Antibody

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Recombinant human full-length SFN protein

Clone: CPTC-SFN-2

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: PC-3 cells, Skin and tissues enriched in stratified squamous keratinizing epithelium. HaCaT, hTCEpi, A-431 cell lines.

Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: SFN (SFN Products)

Synonyms: YWHAS antibody, Er antibody, Mme1 antibody, Ywhas antibody, stratifin antibody, SFN antibody, Sfn antibody

Background: SFN (stratifin) is a p53-induced tumor suppressor gene which is activated in response to DNA damage, causing cell cycle arrest at G2 phase by blocking cdc2-cyclin B1 complex from entering the nucleus. It is inactivated in breast, lung, prostate, liver and gastric cancer. It is associated with poor prognosis when its down-regulation is observed in epithelial ovarian cancer. SFN expression could contribute to cancer cell proliferation and the development and/or progression of human gastrointestinal cancer.

Molecular Weight: 28kDa

Gene ID: 2810

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction