GJB1 antibody

Catalog No. ABIN6939516

Product Details anti-GJB1 Antibody

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GJB1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Specificity: This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called 'connexons' that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane therefore, one connexin usually cannot fully substitute for another.

Immunogen: Recombinant human GJB1 protein

Clone: GJB1-1753

Isotype: IgG

Application Details

Application Notes:

Positive Control: MCF-7 cells. Liver, Kidney, Stomach or Tonsil.

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: GJB1 (GJB1 Products)

Molecular Weight: 27-32kDa

Gene ID: 2705

UniProt: P08034

Pathways: Cell-Cell Junction Organization