Recombinant GFAP antibody
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- Target See all GFAP Antibodies
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human, Rat, Mouse, Pig, Cow, Chicken, Rabbit
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This GFAP antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)
- Specificity
- This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant human full-length GFAP protein
- Clone
- ASTRO-1974R
- Isotype
- IgG
- Top Product
- Discover our top product GFAP Primary Antibody
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- Application Notes
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Positive Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).
Known Application: Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
- Alternative Name
- GFAP (GFAP Products)
- Molecular Weight
- ~50kDa
- Gene ID
- 2670
- UniProt
- P14136
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