Filaggrin antibody (AA 998-1104)

Catalog No. ABIN6939385

Product Details anti-Filaggrin Antibody

Target: Filaggrin (FLG)

Binding Specificity: AA 998-1104

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Filaggrin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Recombinant human Filaggrin protein fragment (around aa 998-1104) (exact sequence is proprietary)

Clone: FLG-1563

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: U-251-MG cells. Skin.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Filaggrin

Target: Filaggrin (FLG)

Alternative Name: FLG (FLG Products)

Synonyms: AW107830 antibody, ft antibody, FLG antibody, ATOD2 antibody, Pflg antibody, Flg antibody, filaggrin antibody, Flg antibody, FLG antibody, LOC100351762 antibody, LOC100728006 antibody

Background: Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Molecular Weight: 26-45kDa (Processed), 350kDa (Profilaggrin)

Gene ID: 2312

UniProt: P20930

Pathways: Sensory Perception of Sound, Stem Cell Maintenance