FAT Atypical Cadherin 2 (FAT2) antibody
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- Target See all FAT Atypical Cadherin 2 (FAT2) Antibodies
- FAT Atypical Cadherin 2 (FAT2)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)
- Specificity
- Recognizes a protein of 480 kDa, which is identified as FAT2. The cadherins represent a family of Ca2+-dependent adhesion molecules that function to mediate cell-to-cell binding that is critical for the maintenance of structure and morphogenesis. Cadherins each contain a large extracellular domain at the N-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short C-terminal intracellular domain interacts with a variety of cytoplasmic proteins, including -catenin, to regulate cadherin function. The cadherin superfamily includes cadherins, protocadherins, desmogleins and desmocollins. FAT2 (FAT tumor suppressor homolog 2) is a single-pass type I membrane protein that belongs to the protocadherin subfamily of cadherins. FAT2 contains one Laminin G-like domain, two EGF-like domains and 32 cadherin domains and is believed to function as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development.
- Purification
- Purified by Protein A/G
- Immunogen
- Purified recombinant human FAT2 fusion protein.
- Clone
- 8C5
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product FAT2 Primary Antibody
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- Application Notes
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Positive Control: Esophagus, Cervix, Uterus, Tonsil or Cerebellum.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- FAT Atypical Cadherin 2 (FAT2)
- Alternative Name
- FAT2 (FAT2 Products)
- Synonyms
- EMI2 antibody, Fath2 antibody, Gm523 antibody, mKIAA0811 antibody, CDHF8 antibody, CDHR9 antibody, HFAT2 antibody, MEGF1 antibody, FAT atypical cadherin 2 antibody, Fat2 antibody, FAT2 antibody
- Molecular Weight
- 480kDa
- Gene ID
- 2196
- UniProt
- Q9NYQ8
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