Estrogen Receptor alpha antibody (AA 129-312)
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- Target See all Estrogen Receptor alpha (ESR1) Antibodies
- Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
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Binding Specificity
- AA 129-312
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Estrogen Receptor alpha antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)
- Specificity
- This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant human ESR1 protein fragment (around aa129-312) (exact sequence is proprietary)
- Clone
- ESR1-3559
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product ESR1 Primary Antibody
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- Application Notes
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Positive Control: MCF-7 cells. Breast or Endometrial Carcinoma (IHC).
Known Application: Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
- Alternative Name
- ESR1 (ESR1 Products)
- Synonyms
- LOC398734 antibody, AA420328 antibody, AU041214 antibody, ER-alpha antibody, ER[a] antibody, ERa antibody, ERalpha antibody, ESR antibody, Estr antibody, Estra antibody, Nr3a1 antibody, NR3A1 antibody, eralpha antibody, zfER[a] antibody, ER antibody, ESRA antibody, ESTRR antibody, Era antibody, akap12 antibody, cb27 antibody, esr1 antibody, fb72g12 antibody, id:ibd1202 antibody, sb:cb27 antibody, si:ch73-192g21.1 antibody, wu:fb72g12 antibody, wu:fc21c03 antibody, Esr antibody, RNESTROR antibody, ERALPHA antibody, er antibody, esr antibody, nr3a1 antibody, XER antibody, era antibody, esra antibody, ERbeta antibody, xesr-1 antibody, xlERalpha1 antibody, xlERalpha2 antibody, ESR1 antibody, estrogen receptor 1 L homeolog antibody, estrogen receptor 1 (alpha) antibody, estrogen receptor 1 antibody, A kinase (PRKA) anchor protein 12b antibody, esr1.L antibody, Esr1 antibody, ESR1 antibody, esr1 antibody, akap12b antibody
- Molecular Weight
- ~67kDa
- Gene ID
- 2099
- UniProt
- P03372
- Pathways
- Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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