Estrogen Receptor alpha antibody

Catalog No. ABIN6939339

Product Details anti-Estrogen Receptor alpha Antibody

Key Features:

• High quality Estrogen Receptor alpha Primary Antibody for the detection of ESR1.
• Reliable product with high standard validation data

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Specificity: This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human Estrogen Receptor alpha protein

Clone: ESR1-1904

Isotype: IgG2a kappa

Product Specific Information:

What can the Estrogen Receptor alpha Antibody ABIN6939339 be used for?: This monoclonal Estrogen Receptor alpha Antibody detects Estrogen Receptor alpha. The Estrogen Receptor alpha Antibody has been validated for various applications and can be used for the detection of Estrogen Receptor alpha and derivatives by Western Blotting, Immunohistochemistry, Staining Methods.

What validation data is available for this Estrogen Receptor alpha Antibody?: The primary antibody has currently 6 product images that show its performance in a variety of applications. The product is currently available in 20 μg, 100 μg quantities. Estrogen Receptor alpha Antibody for the detection of Estrogen Receptor alpha and derivatives.

What is the function of Estrogen Receptor alpha?: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq, Jul 2008].

Application Details

Application Notes:

Positive Control: MCF-7 cells. Breast or Endometrial Carcinoma.

Known Application: Western Blot (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ESR1 (ESR1 Products)

Molecular Weight: ~67kDa

Gene ID: 2099

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly