Emerin antibody (AA 56-167)

Catalog No. ABIN6939303

Product Details anti-Emerin Antibody

Target: Emerin (EMD)

Binding Specificity: AA 56-167

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Emerin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Recombinant human Emerin protein fragment (around aa 56-167) (exact sequence is proprietary)

Clone: EMD-2167

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: K-562, HeLa or Jurkat cells. Kidney or Skin.

Known Application: Western Blot (0.5-2 μg/mL), Immunofluorescence (1-3 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Emerin

Target: Emerin (EMD)

Alternative Name: EMD (EMD Products)

Background: Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma.

Molecular Weight: 37kDa

Gene ID: 2010

UniProt: P50402