Desmoglein 3 antibody
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- Target See all Desmoglein 3 (DSG3) Antibodies
- Desmoglein 3 (DSG3)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Desmoglein 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)
- Specificity
- Recognizes a protein of 130 kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant human DSG3 extracellular protein fragment
- Clone
- 5G11
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product DSG3 Primary Antibody
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- Application Notes
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Positive Control: A431 cells. Lung squamous cell carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Desmoglein 3 (DSG3)
- Alternative Name
- DSG3 (DSG3 Products)
- Synonyms
- DSG3 antibody, CDHF6 antibody, PVA antibody, bal antibody, balding antibody, desmoglein 3 antibody, DSG3 antibody, Dsg3 antibody
- Molecular Weight
- 130kDa
- Gene ID
- 1830
- UniProt
- P32926
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