Desmoglein 3 antibody (AA 379-491)

Catalog No. ABIN6939268

Product Details anti-Desmoglein 3 Antibody

Target: Desmoglein 3 (DSG3)

Binding Specificity: AA 379-491

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Specificity: Recognizes a protein of 130 kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80 % ) and specificity (100 %) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis.

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 379-491) human DSG3 protein (exact sequence is proprietary)

Clone: DSG3-2840

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: A431 cells. Skin or Lung Squamous Cell Carcinoma (SCC).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Desmoglein 3

Target: Desmoglein 3 (DSG3)

Alternative Name: DSG3 (DSG3 Products)

Synonyms: DSG3 antibody, CDHF6 antibody, PVA antibody, bal antibody, balding antibody, desmoglein 3 antibody, DSG3 antibody, Dsg3 antibody

Molecular Weight: 130kDa

Gene ID: 1830

UniProt: P32926