Dystrophin antibody (AA 114-263)

Catalog No. ABIN6939245

Product Details anti-Dystrophin Antibody

Target: Dystrophin (DMD)

Binding Specificity: AA 114-263

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dystrophin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: A recombinant fragment (around aa 114-263) of human DMD protein (exact sequence is proprietary)

Clone: DMD-3245

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: Human skeletal muscle and heart muscle tissues (IHC).

Known Application: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Dystrophin

Target: Dystrophin (DMD)

Alternative Name: DMD (DMD Products)

Background: Dystrophin-glycoprotein complex (DGC) connects the F-Actin cytoskeleton on the inner surface of muscle fibers to the surrounding extracellular matrix, through the cell membrane interface. A deficiency in this protein contributes to Duchenne (DMD) and Becker (BMD) muscular dystrophies. The human dystrophin gene measures 2.4 megabases, has more than 80 exons, produces a 14 kb mRNA and contains at least 8 independent tissue-specific promoters and 2 poly A sites. The dystrophin mRNA can undergo differential splicing and produce a range of transcripts that encode a large set of proteins. Dystrophin represents approximately 0.002 % of total striated muscle protein and localizes to triadic junctions in skeletal muscle, where it is thought to influence calcium ion homeostasis and force transmission.

Molecular Weight: 427kDa

Gene ID: 1756

UniProt: P11532

Pathways: Skeletal Muscle Fiber Development