CLTA/CLTB antibody (N-Term)

Catalog No. ABIN6939151

Product Details anti-CLTA/CLTB Antibody

Target: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLTA/CLTB antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Specificity: Recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.

Purification: Purified by Protein A/G

Immunogen: Purified recombinant N-terminal fragment of human Clathrin Light Chain

Clone: SPM174

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Placenta or Prostate Carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CLTA/CLTB

Target: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Alternative Name: CLTA and CLTB (CLTA/CLTB Products)

Synonyms: lca antibody, zgc:73358 antibody, wu:fb61d08 antibody, wu:fj48d06 antibody, LCA antibody, clc antibody, DDBDRAFT_0169251 antibody, DDBDRAFT_0191305 antibody, DDB_0169251 antibody, DDB_0191305 antibody, clcb antibody, clathrin light chain A antibody, clathrin, light chain A antibody, Clathrin light chain antibody, clathrin light chain antibody, clathrin light chain B antibody, Clathrin light chain B antibody, CLTA antibody, clta antibody, clic-1 antibody, CLC1 antibody, clc antibody, CLTB antibody, clcb antibody, CNI00580 antibody, LOC5567495 antibody, CpipJ_CPIJ018056 antibody, PTRG_01988 antibody, PMAA_096950 antibody, SJAG_04517 antibody, TSTA_044110 antibody, PAS_chr1-4_0158 antibody

Molecular Weight: 31-44kDa

Gene ID: 1211, 1212

UniProt: P09496, P09497