LI Cadherin antibody (AA 242-418)

Catalog No. ABIN6939029

Product Details anti-LI Cadherin Antibody

Target: LI Cadherin

Binding Specificity: AA 242-418

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LI Cadherin antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS)

Specificity: It recognizes a protein of 120 kDa, which is identified as Cadherin 17 (also known as LI Cadherin). The cadherins are a family of Calcium-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. LI-cadherin (for liver-intestine-cadherin) expression is restricted to liver and intestine tissues and is specifically localized to the basolateral domain of hepatocytes and enterocytes.

Cross-Reactivity (Details): Human,

Purification: 200ug/ml of Ab Purified from rabbit anti-serum by Protein A.

Immunogen: Recombinant fragment (around aa 242-418) of human Cadherin 17 protein (CDH17) (exact sequence is proprietary)

Clone: CDH17-2618

Isotype: IgG2b

Application Details

Application Notes:

Positive Control: MCF-7, HepG2 or HT29 cells. Stomach, Small Intestine, Colon or liver.

Known Application: Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for LI Cadherin

Target: LI Cadherin

Alternative Name: CDH17 (LI Cadherin Products)

Synonyms: CDH16 antibody, HPT-1 antibody, HPT1 antibody, HPT-1/LI antibody, cb903 antibody, fb65d02 antibody, wu:fb65d02 antibody, cadherin-17 antibody, cadherin 17 antibody, cadherin 17, LI cadherin (liver-intestine) antibody, CDH17 antibody, Cdh17 antibody, cdh17 antibody

Background: BILL-cadherin, Cadherin-17, CDH17, HPT-1 cadherin, human intestinal peptide-associated transporter HPT-1, human peptide transporter 1 (HPT-1), Intestinal peptide-associated transporter HPT-1, LI-cadherin (liver-intestine), Liver Cadherin, Liver-intestine cadherin,Cadherin 17 / LI Cadherin (Liver-Intestine Marker)
Cellular localisation: Cell Surface and Cytoplasmic

Molecular Weight: 120kDa

Gene ID: 1015, 591853

UniProt: Q12864