Cadherin-16 antibody

Catalog No. ABIN6939019

Product Details anti-Cadherin-16 Antibody

Target: Cadherin-16 (CDH16)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cadherin-16 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Specificity: This MAb recognizes a protein of 130 kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

Cross-Reactivity (Details): Human, Mouse, Rat, Rabbit, Dog,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human CDH16 protein

Clone: CDH16-1071

Isotype: IgG1

Application Details

Application Notes:

Positive Control: HEK293 cells. Kidney tissue lysate. Normal kidney or renal cell carcinoma.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Flow Cytometry (1-2 μg/million cells),Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for Cadherin-16

Target: Cadherin-16 (CDH16)

Alternative Name: CDH16 (CDH16 Products)

Synonyms: cadherin 16 antibody, CDH16 antibody, Cdh16 antibody

Background: Cadherin-16 (CDH16), Kidney-specific cadherin, Ksp-cadherin antibody,Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16
Cellular localisation: Cell Surface. Some cytoplasmic.

Molecular Weight: 130kDa

Gene ID: 1014, 513660

UniProt: O75309