XRCC6/XRCC5 antibody
-
- Target See all XRCC6/XRCC5 products
- XRCC6/XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5/6 (XRCC6/XRCC5))
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This XRCC6/XRCC5 antibody is un-conjugated
-
Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Specificity
- Recognizes a dimer of two proteins of 70 kDa and ~80 kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
- No Cross-Reactivity
- Chicken, Mouse (Murine), Rat (Rattus)
- Cross-Reactivity (Details)
- Human. Primates. Does not react with mouse, rat and chicken.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Nuclear extract of human HL-60 cells
- Clone
- KU729
- Isotype
- IgG1 kappa
-
-
- Application Notes
-
Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunocytochemistry (Acetone-fixed cells) (0.5-1.0 μg/mL for 30 minutes at RT),Optimal dilution for a specific application should be determined.
Positive_Control: K562, HL-60, HEp-2 or HeLa cells.
- Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
-
- Target
- XRCC6/XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5/6 (XRCC6/XRCC5))
- Alternative Name
- XRCC6 (p70) & XRCC5 (p80) (XRCC6/XRCC5 Products)
- Background
-
70 kDa / 80 kDa subunit of Ku antigen, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, G22P1, Lupus Ku autoantigen protein p70, ML8, Thyroid autoantigen 70kD (Ku antigen), Thyroid-lupus autoantigen (TLAA), X-ray repair cross-complementing protein 6 (XRCC6), ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, KARP1, KUB2, Lupus Ku autoantigen protein p86, Nuclear factor IV (NFIV), X-ray repair cross-complementing protein 5 (XRCC5),Ku (p70/p80) (Nuclear Marker)
Cellular localisation: Nuclear - Molecular Weight
- 70kDa & 80kDa
-