VWF antibody (AA 845-949)

Catalog No. ABIN6938770

Product Details anti-VWF Antibody

Target: VWF (Von Willebrand Factor (VWF))

Binding Specificity: AA 845-949

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VWF antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human vWF fragment spanning around aa 845-949 (exact sequence is proprietary)

Clone: 3E2D10

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein lysate),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HUVEC cells. Tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for VWF

Target: VWF (Von Willebrand Factor (VWF))

Alternative Name: VWF (VWF Products)

Synonyms: VWF antibody, si:ch1073-474e24.1 antibody, F8VWF antibody, VWD antibody, 6820430P06Rik antibody, AI551257 antibody, B130011O06Rik antibody, C630030D09 antibody, von Willebrand factor antibody, Von Willebrand factor antibody, VWF antibody, vwf antibody, Vwf antibody

Background: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD),von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 250kDa

Gene ID: 7450, 440848

UniProt: P04275