PPP3R1 antibody

Catalog No. ABIN6938230

Product Details anti-PPP3R1 Antibody

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPP3R1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59 kDa catalytic A subunit and a 19 kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length Calcineurin B protein

Clone: CALNB-2342

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF-7 or A431 cells. Prostate, Thyroid or Pancreas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PPP3R1

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Alternative Name: PPP3R1 (PPP3R1 Products)

Synonyms: si:ch211-274c22.8 antibody, canb antibody, CALNB1 antibody, CNB antibody, CNB1 antibody, CaNB1 antibody, Cnb1 antibody, MCIP1 antibody, protein phosphatase 3, regulatory subunit B, alpha a antibody, protein phosphatase 3 regulatory subunit B, alpha S homeolog antibody, protein phosphatase 3 regulatory subunit B, alpha antibody, protein phosphatase 3, regulatory subunit B, alpha antibody, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) antibody, ppp3r1a antibody, ppp3r1.S antibody, PPP3R1 antibody, Ppp3r1 antibody

Background: Calcineurin B, type I (19 kDa), Calcineurin subunit B type 1, CALNB1, CNB1, PPP3R1 protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform, Protein phosphatase 3 regulatory subunit B alpha, Protein phosphatase 3 regulatory subunit B alpha isoform 1,Calcineurin B / PPP3R1
Cellular localisation: Cytoplasmic and Cell Surface

Molecular Weight: 19kDa

Gene ID: 5534, 280604

UniProt: P63098

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling