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Keratin 5/8 antibody

KRT5/8 Reactivity: Human WB, FACS, IF, IHC (f) Host: Mouse Monoclonal C-50 unconjugated
Catalog No. ABIN6937713
  • Target See all Keratin 5/8 (KRT5/8) products
    Keratin 5/8 (KRT5/8)
    Reactivity
    • 22
    • 16
    • 15
    • 13
    • 13
    • 13
    • 12
    • 10
    • 9
    • 3
    • 2
    • 2
    Human
    Host
    • 22
    Mouse
    Clonality
    • 22
    Monoclonal
    Conjugate
    • 9
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Keratin 5/8 antibody is un-conjugated
    Application
    • 16
    • 15
    • 13
    • 12
    • 8
    • 7
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificity
    It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
    No Cross-Reactivity
    Chicken, Xenopus laevis
    Cross-Reactivity (Details)
    Human. Pig. Mouse. Rat. Hamster. Cow. Dog. Sheep. Ferret. Does not react with Chicken or Xenopus.
    Immunogen
    Cytoskeletal preparation from HeLa cells
    Clone
    C-50
    Isotype
    IgG1 kappa
  • Application Notes

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.

    Positive_Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Preservative
    Azide free
    Storage
    4 °C
    Storage Comment
    Store at 2 to 8°C. Antibody is stable for 24 months.
    Expiry Date
    24 months
  • Target
    Keratin 5/8 (KRT5/8)
    Alternative Name
    KRT5 & KRT8 (KRT5/8 Products)
    Background
    CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8,Cytokeratin 5/8
    Cellular localisation: Cytoplasmic
    Molecular Weight
    58kDa (CK5), 52.5kDa (CK8)
    Gene ID
    3852, 3856, 433845
    UniProt
    P13647, P05787
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