Keratin 5/8 antibody
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- Target See all Keratin 5/8 (KRT5/8) products
- Keratin 5/8 (KRT5/8)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Keratin 5/8 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
- No Cross-Reactivity
- Chicken, Xenopus laevis
- Cross-Reactivity (Details)
- Human. Pig. Mouse. Rat. Hamster. Cow. Dog. Sheep. Ferret. Does not react with Chicken or Xenopus.
- Immunogen
- Cytoskeletal preparation from HeLa cells
- Clone
- C-50
- Isotype
- IgG1 kappa
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- Application Notes
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.
Positive_Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Preservative
- Azide free
- Storage
- 4 °C
- Storage Comment
- Store at 2 to 8°C. Antibody is stable for 24 months.
- Expiry Date
- 24 months
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- Target
- Keratin 5/8 (KRT5/8)
- Alternative Name
- KRT5 & KRT8 (KRT5/8 Products)
- Background
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CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8,Cytokeratin 5/8
Cellular localisation: Cytoplasmic - Molecular Weight
- 58kDa (CK5), 52.5kDa (CK8)
- Gene ID
- 3852, 3856, 433845
- UniProt
- P13647, P05787
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