CD25 antibody
-
- Target See all CD25 (IL2RA) Antibodies
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This CD25 antibody is un-conjugated
-
Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)
- Specificity
- Recognizes a protein of 55kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin's disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human IL2RA protein
- Clone
- IL2RA-423
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product IL2RA Primary Antibody
-
-
- Application Notes
-
Known_Application: Functional Studies (Order Ab without Azide), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.
Positive_Control: PHA-stimulated human lymphocytes. Human lymph nodes and tonsils.
- Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
-
- Target
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Alternative Name
- IL2RA (IL2RA Products)
- Synonyms
- CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody
- Background
-
IL2 Receptor alpha, IL2R alpha chain, IL2R, IL2R subunit alpha IL2RA, Interleukin-2 receptor subunit alpha, Ly43, p55, p55 chain, T Cell Growth Factor Receptor, TAC antigen, TCGFR,CD25 / IL2RA (Activated Lymphocyte Marker)
Cellular localisation: Cell Surface - Molecular Weight
- 55kDa
- Gene ID
- 3559, 231367
- UniProt
- P01589
- Pathways
- JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
-