IGHG4 antibody

Catalog No. ABIN6937504

Product Details anti-IGHG4 Antibody

Target: IGHG4 (Immunoglobulin Heavy Constant gamma 4 (G4m Marker) (IGHG4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGHG4 antibody is un-conjugated

Application: Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: The regions of relatively constant sequence beyond the variable regions of immunoglobulins are termed constant regions (C regions) and are present in both the heavy and light chains. With very few exceptions, the sites of attachment for carbohydrates on immunoglobulins are located in these C regions. These regions also function to hold the variable regions together by using the disulfide bond between them. The C regions facilitate interaction with the antigen by increasing the maximum rotation of the immunoglobulin arms. Reportedly, a large population of patients with recurrent respiratory tract infection has low IgG4 concentrations. IgG4-related sclerosing disease has been recognized as a systemic disease entity characterized by an elevated serum IgG4 level, sclerosing fibrosis, and diffuse lymphoplasmacytic infiltration with the presence of many IgG4-positive plasma cells.IgG4 is overexpressed in inflammatory pseudotumor (IPT) and under expressed in inflammatory myofibroblastic tumor (IMT). In pulmonary nodular lymphoid hyperplasia (PNLH), there are an increased number of IgG4+ plasma cells.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human IGHG4 fragment

Clone: IGHG4-1345

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for IGHG4

Target: IGHG4 (Immunoglobulin Heavy Constant gamma 4 (G4m Marker) (IGHG4))

Alternative Name: IGHG4 (IGHG4 Products)

Synonyms: immunoglobulin heavy constant gamma 4 (G4m marker) antibody, IGHG4 antibody

Background: Ig gamma 4 chain C region, IGHG4, Immunoglobulin heavy constant gamma 4 (G4m marker),IgG4 (Ig Heavy Constant Gamma 4) (G4m Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 150kDa

Gene ID: 3503, 510635

UniProt: P01861