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IGHG antibody

IGHG Reactivity: Human IHC, FACS, IF, IHC (p) Host: Mouse Monoclonal IG217 unconjugated
Catalog No. ABIN6937497
  • Target See all IGHG products
    IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))
    Reactivity
    • 15
    • 2
    Human
    Host
    • 8
    • 7
    • 2
    Mouse
    Clonality
    • 15
    • 2
    Monoclonal
    Conjugate
    • 15
    • 1
    • 1
    This IGHG antibody is un-conjugated
    Application
    • 10
    • 10
    • 7
    • 6
    • 6
    • 4
    • 4
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
    Cross-Reactivity (Details)
    Human.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogen
    Purified human Ig Gamma Chain
    Clone
    IG217
    Isotype
    IgG1 kappa
  • Application Notes

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin/paraffin) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Preservative
    Azide free
    Storage
    -20 °C,-80 °C
    Storage Comment
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Expiry Date
    24 months
  • Target
    IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))
    Alternative Name
    IGHG (IGHG Products)
    Synonyms
    AU044919 antibody, IGHG2A antibody, Immunoglobulin heavy chain (gamma polypeptide) antibody, Ighg antibody
    Background
    G1m Marker, G2m Marker, G3m Marker, G4m Marker, HDC, Heavy Chain Disease Protein, Human Immunglobulin G, Ig gamma1/2/3/4 Chain C Region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobulin Heavy Constant 1/2/3/4,IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker)
    Cellular localisation: Cytoplasm, Cell Surface and Secreted
    Molecular Weight
    75kDa
    Gene ID
    3500, 3501, 3502
    UniProt
    P01857, P01859, P01860, P01861
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