Recombinant HLA DQ antibody

Catalog No. ABIN6937408

Product Details anti-HLA DQ Antibody

Target: HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HLA DQ antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.

Cross-Reactivity (Details): Human. Pig.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human HLA-DQ protein

Clone: HLA-DQA1-2866R

Isotype: IgG

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Frozen only) (1-2 μg/mL for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Positive_Control: Raji cells. Tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for HLA DQ

Target: HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))

Alternative Name: HLA-DQ (HLA-DQ Products)

Background: CELIAC1, DC 4 alpha chain Gluten sensitive enteropathy (celiac disease) (GSE), HLA class II histocompatibility antigen DQ(1) alpha chain, HLA class II histocompatibility antigen DQ(3) alpha chain, HLA DQA1, HLA DQB1, IDDM1, Leucocyte antigen DQA1, Major histocompatibility complex class II DQ alpha 1, Major histocompatibility complex class II DQ beta 1,HLA-DQ (MHC II)
Cellular localisation: Cell Surface

Molecular Weight: 60kDa

Gene ID: 3117, 3118, 3119

UniProt: P01908, P01909, P01920