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MHC Class II HLA-DP/DQ/DR antibody

HLA-DP/DQ/DR Reactivity: Human FACS, IF, WB, Coat, ELISA, IHC (f) Host: Mouse Monoclonal CR3-43 unconjugated
Catalog No. ABIN6937400
  • Target See all MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR) products
    MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
    Reactivity
    • 18
    • 1
    Human
    Host
    • 16
    • 2
    Mouse
    Clonality
    • 18
    Monoclonal
    Conjugate
    • 10
    • 4
    • 2
    • 2
    This MHC Class II HLA-DP/DQ/DR antibody is un-conjugated
    Application
    • 16
    • 7
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Coating (Coat), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificity
    Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
    Cross-Reactivity (Details)
    Human.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogen
    Cells from human tonsil
    Clone
    CR3-43
    Isotype
    IgG1 kappa
  • Application Notes

    Known_Application: ELISA (For coating use Ab at 2-4 μg/mL, order Ab without BSA),Flow Cytometry (1-2 μg/ million cells), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Ramos, Raji cells. Tonsil, Spleen or lymph node.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
    Preservative
    Azide free
    Storage
    -20 °C,-80 °C
    Storage Comment
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Expiry Date
    24 months
  • Target
    MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
    Alternative Name
    HLA-DP, HLA-DQ, HLA-DR (HLA-DP/DQ/DR Products)
    Background
    HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5,HLA-DP/-DQ/-DR (MHC II)
    Cellular localisation: Cell Surface
    Molecular Weight
    36kDa (' chain) and 27kDa (' chain)
    Gene ID
    3115, 3117, 3122
    UniProt
    P04440, P01908, P01909, P01920, P01903
    Pathways
    Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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