MHC Class II HLA-DP/DQ/DR antibody
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- Target See all MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR) products
- MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MHC Class II HLA-DP/DQ/DR antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Coating (Coat), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Cells from human tonsil
- Clone
- CR3-43
- Isotype
- IgG1 kappa
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- Application Notes
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Known_Application: ELISA (For coating use Ab at 2-4 μg/mL, order Ab without BSA),Flow Cytometry (1-2 μg/ million cells), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Ramos, Raji cells. Tonsil, Spleen or lymph node.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
- Alternative Name
- HLA-DP, HLA-DQ, HLA-DR (HLA-DP/DQ/DR Products)
- Background
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HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5,HLA-DP/-DQ/-DR (MHC II)
Cellular localisation: Cell Surface - Molecular Weight
- 36kDa (' chain) and 27kDa (' chain)
- Gene ID
- 3115, 3117, 3122
- UniProt
- P04440, P01908, P01909, P01920, P01903
- Pathways
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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