Recombinant MHC Class II HLA-DP/DQ/DR antibody

Catalog No. ABIN6937399

Product Details anti-MHC Class II HLA-DP/DQ/DR Antibody

Target: MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MHC Class II HLA-DP/DQ/DR antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Coating (Coat), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a ' chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten ' chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

Clone: HLA-Pan-2967R

Isotype: IgG

Application Details

Application Notes:

Known_Application: ELISA (For coating use Ab at 2-5 μg/mL, order Ab without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Ramos or Raji cells. Human tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MHC Class II HLA-DP/DQ/DR

Target: MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)

Alternative Name: HLA-DP, HLA-DQ, HLA-DR (HLA-DP/DQ/DR Products)

Background: HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5,HLA-Pan (MHC II)
Cellular localisation: Cell Surface

Molecular Weight: 33-35kDa

UniProt: P01908, P01909

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response