CLTA/CLTB antibody (N-Term)

Catalog No. ABIN6937002

Product Details anti-CLTA/CLTB Antibody

Target: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLTA/CLTB antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Purified recombinant N-terminal fragment of human Clathrin Light Chain

Clone: SPM174

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Placenta or Prostate Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CLTA/CLTB

Target: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Alternative Name: CLTA and CLTB (CLTA/CLTB Products)

Synonyms: lca antibody, zgc:73358 antibody, wu:fb61d08 antibody, wu:fj48d06 antibody, LCA antibody, clc antibody, DDBDRAFT_0169251 antibody, DDBDRAFT_0191305 antibody, DDB_0169251 antibody, DDB_0191305 antibody, clcb antibody, clathrin light chain A antibody, clathrin, light chain A antibody, Clathrin light chain antibody, clathrin light chain antibody, clathrin light chain B antibody, Clathrin light chain B antibody, CLTA antibody, clta antibody, clic-1 antibody, CLC1 antibody, clc antibody, CLTB antibody, clcb antibody, CNI00580 antibody, LOC5567495 antibody, CpipJ_CPIJ018056 antibody, PTRG_01988 antibody, PMAA_096950 antibody, SJAG_04517 antibody, TSTA_044110 antibody, PAS_chr1-4_0158 antibody

Background: Clathrin light chain A, Clathrin light chain B, Clathrin light chain LCA, Clathrin light chain LCB, Clathrin light polypeptide A, Clathrin light polypeptide B, Clathrin, light polypeptide (Lca), Clathrin, light polypeptide (Lcb), CLTA, CLTB,Clathrin, Light Chain
Cellular localisation: Cytoplasmic

Molecular Weight: 31-44kDa

Gene ID: 1211, 1212, 484241

UniProt: P09496, P09497