CD14 antibody
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- Target See all CD14 Antibodies
- CD14
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD14 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF)
- Specificity
- Recognizes a protein of 55 kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant human CD14 protein
- Clone
- LPSR-927
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD14 Primary Antibody
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- Application Notes
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.
Positive_Control: THP-1 cells and human monocytes. Lymph nodes and tonsils.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- CD14
- Alternative Name
- CD14 (CD14 Products)
- Synonyms
- CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody
- Background
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Lipopolysaccharide receptor, LPS-R, LPSR, Mo2, Monocyte Differentiation Antigen 14, Myeloid cell-specific leucine-rich glycoprotein,CD14 (Monocyte / Macrophage Marker)
Cellular localisation: Cell Surface - Molecular Weight
- 55kDa
- Gene ID
- 929, 163867
- UniProt
- P08571
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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